Product Info Summary
SKU: | A01977 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-LIM domain-binding protein 1 LDB1 Antibody
SKU/Catalog Number
A01977
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-LIM domain-binding protein 1 LDB1 Antibody (Catalog # A01977). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-LIM domain-binding protein 1 LDB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01977)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01977 is reactive to LDB1 in Human
Applications
A01977 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
46.533kDa
Background of LDB1
LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:425,000
ChIP: User optimized
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of LDB1 expression in Jurkat whole cell lysate (lane 1). LDB1 was detected using rabbit anti-LDB1 Antigen Affinity purified polyclonal antibody (Catalog # A01977) at 1:1800. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For LDB1 (Source: Uniprot.org, NCBI)
Gene Name
LDB1
Full Name
LIM domain-binding protein 1
Weight
46.533kDa
Superfamily
LDB family
Alternative Names
carboxy terminal LIM domain protein 2; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; CLIM2hLdb1; LDB-1; LIM domain binding 1; LIM domain-binding factor-1; LIM domain-binding protein 1; NLILIM domain-binding factor CLIM2; Nuclear LIM interactor LDB1 CLIM-2, CLIM2, LDB-1, NLI LIM domain binding 1 LIM domain-binding protein 1|LIM domain-binding factor CLIM2|LIM domain-binding factor-1|carboxy terminal LIM domain protein 2|carboxyl-terminal LIM domain-binding protein 2|nuclear LIM interactor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LDB1, check out the LDB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LDB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIM domain-binding protein 1 LDB1 Antibody (A01977)
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No publications found for A01977
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-LIM domain-binding protein 1 LDB1 Antibody
Question
I have attached the WB image, lot number and protocol we used for right ovary using anti-LDB1 antibody A01977. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-01
Question
Would anti-LDB1 antibody A01977 work for WB with right ovary?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of right ovary, LDB1 is highly expressed in right ovary. So, it is likely that anti-LDB1 antibody A01977 will work for WB with right ovary.
Boster Scientific Support
Answered: 2019-08-20
Question
We are currently using anti-LDB1 antibody A01977 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
A. Krishna
Verified customer
Asked: 2017-11-21
Answer
The anti-LDB1 antibody (A01977) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-21